HPLC Method for Analysis of Deoxyuridine on Chromite™ Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Deoxyuridine
Deoxyuridine ( dU ) is an nucleoside with the C9H12N2O5molecular formula. While in living organisms it exists primarily to occasionally become a part of DNA, it’s variants, Idoxuridine and Trifluoride, work as antiviral drugs. If consumed, Deoxyuridine can be toxic.
Deoxyuridine can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Methodenparameter
| Column | Chromni, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 90% |
| Buffer | AmFm pH 3.0 – 30 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |