HPLC Method for Analysis of Biopterin on Primesep 100 Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Biopterin
Biopterin is a petrin derivative with the chemical formula C9H11N5O3 . As a cofactor for aromatic amino acid hydroxylases, it is a part of the synthesis of neurotransmitters like dopamine, serotonin, and norepinephrine. A biopterin imbalance can lead to a variety of disorders, most of which lead to neurological problems.
Biopterin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |