HPLC Method for Analysis of Acetaminophen on Primesep 100 Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetaminophen (Paracetamol)
Acetaminophen , also known as Paracetamol, is a medication with the molecular formula C8H9NO2 . It is an over-the-counter pain killer that also reduces fever. Unlike Ibuprofen and naproxen, it is not an anti-inflammatory drug.
Acetaminophen (Paracetamol) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 10% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |