HPLC Method For Analysis Of 2,4,6-Trichloroanisole on Newcrom R1 Column on Alltesta™

Separation type: Liquid Chromatography Reverse Phase

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2,4,6-Trichloroanisole

2,4,6-Trichloroanisole (TCA) is a fungal metabolite of 2,4,6-Trichlorophenol, which is a popular fungicide. TCA also has a distinct musty smell that is most evident when wines develop tainted corks. TCA can be retained and analyzed on a Newcrom R1 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and no buffer. This analysis method can be UV detected at 210 nm.

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A , AH , B , and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.