HPLC Determination of Piracetam on SHARC 1 Column

HPLC-Applikation • Datum: 2021/04/01
HPLC Determination of Piracetam on SHARC 1 Column Chromatogramm

High Performance Liquid Chromatography (HPLC) Method for Analysis of Piracetam

Piracetam has been marketed as a treatment for myoclonus, vertigo, dyslexia, and sickle cell anemia. It is approved for use across many European countries, but is not approved by the FDA in the United States. It’s chemical formula is C6H10N2O2 .

[compound] can be detected in the low UV regime. Using a SHARC1hydrogen-bonding column and a mobile phase consisting of acetonitrile (MeCN) and water without a buffer, Piracetam can be retained, measured, and analyzed. This method can be UV detected at 200 nm with very high resolution

Methodenparameter

ColumnSHARC1, 4.6 x 150 mm, 5 µm, 100 Å, dual ended
Mobile PhaseMeCN/H2O -98/2%
BufferNo
Flow Rate1.0 mL/min
DetectionUV 200 nm