HPLC Determination of Dicyandiamide on Sharc 1 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Dicyandiamide
Dicyandiamide, also known as cyanoguanidine, has a C2H4N4chemical formula. It is a white crystalline powder that has a variety of uses from agricultural to mechanical. Though, typically it is found in fertilizers as a nitrification inhibitor. It slows down nitrogen release and and reduces losses from soil.
Dicyandiamide can be retained and analyzed using the SHARC1stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a triethanolamine buffer. Detection is performed using CAD.
Methodenparameter
| Column | SHARC1, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 97.8% |
| Buffer | AmFm 0.2%, Formic Acid – 2% |
| Flow Rate | 1.0 mL/min |
| Detection | CAD (Corona) MS- compatible mobile phase |