HPLC Determination of Caffeine, Theophylline and Theobromine in Tea on Primesep SB Column

HPLC-Applikation • Datum: 2020/12/17
HPLC Determination of Caffeine, Theophylline and Theobromine in Tea on Primesep SB Column Chromatogramm

Diese HPLC-Applikation beschreibt die chromatographische Analyse zu „HPLC Determination of Caffeine, Theophylline and Theobromine in Tea on Primesep SB Column“.

Methodenparameter

ColumnPrimesep SB, 3.2 x 100 mm, 5 µm, 100 Å, dual ended
Mobile PhaseEtOH – 10%
BufferH3PO4 – 0.3%
Flow Rate0.5 mL/min
DetectionUV, 270 nm