HPLC Method for Analysis of Sildenafil Citrate (Viagra) in a Tablet Primesep 100 Column
Sildenafil Citrate (also known as Viagra ) is one of the most popular medications used to treat erectile dysfunction and pulmonary hypertension. It works primarily through increasing bloodflow. It has the chemical formula C28H38N6O11S . It is a part of the group called phosphodiesterase 5 (PDE5) inhibitors, which prevent the enzyme called “phosphodiesterase type-5” from working.
Sildenafil or Viagra can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 214 nm and 294 nm with high resolution and peak symmetry.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 60% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | λmax 214, 294 nm |