HPLC Method For Analysis Of Meldonium in Mildronate Tablet Dosage Form on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of L-Carnitine and Arginine
Meldonium is a popular treatment for ischemia in Eastern Europe. It helps open the arteries, thereby increasing blood flow. Due to reports of athletes using it as a performance-enhancing drug, it has been banned by the World Anti-Doping Agency since 2016. Meldonium can be detected in the low UV regime. Using a Primesep 100 reverse-phase column and a mobile phase consisting of water and acetonitrile (MeCN) with a perchloric acid (HClO4) buffer, meldonium can be retained, measured, and analyzed. This analysis method can be UV detected at 205 nm.
Methodenparameter
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | HClO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 205 nm |