High Performance Liquid Chromatography (HPLC) Method for Analysis of Nebivolol , Bystolic .
Nebivolol is a beta-blocker with the chemical formula C22H25F2NO4 . It is an oral medication used to treat high blood pressure, but is not preferred to alternatives. Side effects of it include dizziness, fatigue, nausea, and headaches. You can find detailed UV spectra of Nebivolol , Bystolic and information about its various lambda maxima by visiting the following link.
Nebivolol can be retained in HPLC on Primesep SB reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. UV detection at 285 nm.
| Column | (Column variation not found) |
|---|---|
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 285 nm |