HPLC Method for Analysis of Labetalol (stereoisomers) in Pharmaceutical Dosage Form on Primesep B Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Labetalol is primarily used for the management of hypertension. It is classified as a beta-blocker, which works by blocking the effects of endogenous chemicals such as epinephrine on the heart and blood vessels. Through this mechanism, a reduction in heart rate, blood pressure, and myocardial strain is achieved.
Labetalol can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 230 nm.
You can find detailed UV spectra of Labetalol and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep B, 4.6 x 250 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 15% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 0.7 mL/min |
| Detection | UV 230 nm |