HPLC MS Method for Analysis of Labetalol in Pharmaceutical Dosage Form on Primesep 200 Column
Labetalol is a medication used to treat high blood pressure (hypertension). It is a combined alpha- and beta-blocker, which means it works by blocking both alpha and beta receptors in the body.
Labetalol can be retained, and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Formic Acid as a buffer. Detection is achieved using UV at 300 nm
You can find detailed UV spectra of Labetalol and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep 200, 2.1 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 0.2 mL/min |
| Detection | UV 300 nm, SIM + 329 |