HPLC Method for Analysis of Sertraline in Tablet Dosage on Primesep B Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Sertraline is an antidepressant belonging to the class of selective serotonin reuptake inhibitors (SSRIs). It is commonly prescribed to treat conditions like depression, anxiety disorders, obsessive-compulsive disorder (OCD), panic attacks, and post-traumatic stress disorder (PTSD).
Common Uses Major depressive disorder (MDD) Generalized anxiety disorder (GAD) Panic disorder Obsessive-compulsive disorder (OCD) Post-traumatic stress disorder (PTSD) Social anxiety disorder
Sertraline can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 195 nm.
You can find detailed UV spectra of Sertraline and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 195 nm |
| Limit of Detection | 4 ppb |