HPLC Method for Analysis of Metoprolol in Tablets on Primesep 200 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Metoprolol
Metoprolol is a beta-blocker that is used to treat high blood pressure, angina, and heart failure by blocking beta-1 adrenergic receptors. At it’s most effective, it can lower the risk of death after a heart attack.
Metoprolol can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 223 nm |