HPLC Method for Analysis of Adenine on Primesep 100 Column with Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenine
Adenine is a chemical compound with the molecular formula C5H5N5 . It is a building block for DNA and RNA as one of the four essential nucleotide bases. It is vital as a part of the main energy-carrying molecule in cells. When in DNA, it pairs with thymine, while in RNA, it pairs with uracil.
Adenine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 3.2 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | Sulfuric Acid |
| Flow Rate | 0.5 mL/min |
| Detection | UV 275 nm |