HPLC Method for Analysis of 4-Amino-2-nitrophenol on Primesep 100 Column with Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Amino-2-nitrophenol
4-Amino-2-nitrophenol is a hydrocarbon with the molecular formula C6H6N2O3 . It is a red-purple powder that is typically used in as an oxidation base in hair dye. California considers this compound a carcinogen.
4-Amino-2-nitrophenol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 3.2 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |