HPLC Determination of Tramadol in Human Plasma and Urine Samples with FlipLC system

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tramadol , Tramadol hydrochloride .

FlipLC™ is an alternative method to avoid the interference of most of the contaminants by the use of an isolation column and a high pressure switching valve before the separation column. This method allows sample cleaning and analyte separation in one automated process. The isolation column and the separation column should have orthogonal retention characteristics to operate efficiently in this setup. Mixed-mode columns with reverse phase and ion-exchange characteristics were used in this analysis.