HPLC Determination of 6-Aminocaproic Acid With Low UV Detection
High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Aminocaproic acid .
6-Aminocaproic Acid , brand name Amicar, is a clotting agent used to control excessive bleeding in patients during or after surgery by acting as an inhibitor to fibrinolysis. It is also used to treat a variety of disorders from Thrombocytopenia to bleeding disorders. It’s chemical formula is C6H13NO2 . You can find detailed UV spectra of 6-Aminocaproic Acid and information about its various lambda maxima by visiting the following link.
It can be retained using HPLC on Newcrom AH mixed-mode column using isocratic analysis with mobile phase consisting of acetonitrile and water (ACN/H2O) with sulfuric acid (H2SO4) as buffer and detected at low UV of 200 nm.
Methodenparameter
| Column | Newcrom АH, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |