HPLC Determination of 6-aminocaproic Acid on Newcrom A Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Aminocaproic acid .
6-Aminocaproic Acid , brand name Amicar, is a clotting agent used to control excessive bleeding in patients during or after surgery by acting as an inhibitor to fibrinolysis. It is also used to treat a variety of disorders from Thrombocytopenia to bleeding disorders. It’s chemical formula is C6H13NO2 . You can find detailed UV spectra of 6-Aminocaproic Acid and information about its various lambda maxima by visiting the following link.
It can be retained using HPLC and low capacity Newcrom A mixed-mode column using isocratic analysis with mobile phase consisting of acetonitrile and water (ACN/H2O) and MS-compatible ammonium formate (AmFm) buffer.
Methodenparameter
| Column | Newcrom A, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | Ammonium Formate pH 3.0 – 10 мМ |
| Flow Rate | 1.0 mL/min |
| Detection | CAD, Corona (MS-compatible mobile phase) |