HPLC Method for the Determination of Acetaminophen, Phenylephrine, and Ascorbic Acid on Primesep S Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetaminophen, Phenylephrine, and Ascorbic Acid

Acetaminophen , also known as Paracetamol, is a medication with the molecular formula C8H9NO2 . It is an over-the-counter pain killer that also reduces fever. Unlike Ibuprofen and naproxen, it is not an anti-inflammatory drug.

Phenylephrine is a common over-the-counter decongestant, but can also be used for pupil dilation and hemorrhoid treatment. It’s chemical formula is C9H13NO2 . It is sold under many brand names including Mucinex, Sudafed PE, Sinex, and many other generic brands. While it is best known as a decongestant, it has a wide variety of other pharmaceutical applications, including pupil dilation, hypotension (low blood pressure) treatment, and hemorrhoid relief.

Ascorbic Acid is a vitamin with the molecular formula C6H8O6 . Typically, it is used to treat scurvy, support immune system, and preserve food. It is a white to light yellow powder that is easily dissolved in water. It can be found in a large variety of fruits and vegetables, especially in citrus.

You can find detailed UV spectra of Acetaminophen and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Ascorbic Acid and information about its various lambda maxima by visiting the following link.