HPLC Determination of Tranexamic acid (TXA) on Primesep 200 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tranexamic acid. Tranexamic acid is a synthetic derivative of the amino acid lysine. Tranexamic acid is an antifibrinolytic agent. It works by blocking the breakdown of blood clots, which prevents bleeding.
Tranexamic acid can be retained on a Primesep 200 mixed-mode column with embedded weak acidic ion-pairing groups, having great peak shape using an isocratic method of acetonitrile (ACN), water and phosphoric acid (H3PO4) as a buffer. UV Detection 210 nm.
Methodenparameter
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 210 nm |