HPLC Determination of Octylphosphonic acid on Newcrom A
Octylphosphonic acid ( OPA ) is a surfactant with the chemical formula C8H19O3P . It can be used to synthesize self-assembled monolayers (SAMs), polymeric matrices, and as a coating for super-resolution microscopy.
Using a Newcrom A mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer, OPA can be retained, measured, and analyzed. This analysis method can be used in conjunction with charged aerosol detection (CAD).
Methodenparameter
| Column | Newcrom AH, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | CAD |