Simultaneous Analysis of Glucose, Sodium, Potassium and Calcium Ions

HPLC-Applikation • Datum: 2011/07/08
Simultaneous Analysis of Glucose, Sodium, Potassium and Calcium Ions Chromatogramm

Neutral sugar, monovalent and divalent cations were separated in HILIC/cation-exchange mode on a Primesep N mixed-mode column. Mobile phase is compatible with LC/MS and method can be used for analysis polar molecules in HILIC, cation-exchange or anion-exclusion modes.

Methodenparameter

ColumnPrimesep N, 4.6×150 mm, 5 µm, 100 Å
Mobile PhaseMeCN/H2O
BufferAmAc pH 4.0
Flow Rate1.0 mL/min
DetectionELSD50C