Neutral sugar, monovalent and divalent cations were separated in HILIC/cation-exchange mode on a Primesep N mixed-mode column. Mobile phase is compatible with LC/MS and method can be used for analysis polar molecules in HILIC, cation-exchange or anion-exclusion modes.
| Column | Primesep N, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc pH 4.0 |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD50C |