HPLC Analysis of Calcium Ions in Maple Syrup

HPLC-Applikation • Datum: 2012/07/10
HPLC Analysis of Calcium Ions in Maple Syrup Chromatogramm

Application Notes: maple syrup primarily consists of sucrose and other sugars, but also has small amounts of amino acids (arginine, threonine, proline), organic acids (malic and fumaric), vitamins (niacin and riboflavin) and traces of minerals (Na, K, Ca, Mn, Mg, Zn ions). One analysis performed on maple syrup is calcium ion determination. A Primesep 100 column was used in cation-exchange mode to isolate calcium ion from other components of the maple syrup. This method can be used for quantification of calcium ion by ELSD/CAD. Limit of detection depends on the type of ELSD/CAD detector used but usually reaches 20 ppm

Application Columns: Primesep 100 Application compounds: calcium ions, Maple Syrup Detection technique: LC/MS, ELSD/CAD

Methodenparameter

ColumnPrimesep 100, 4.6×150 mm, 5 µm, 100 Å
Mobile PhaseMeCN/H2O
BufferTFA
Flow Rate1.0 mL/min
DetectionELSD