HPLC ELSD Method for Separation of Metal Ions on Primesep 100 Column
Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.
Methodenparameter
| Column | Primesep 100, 4.6×250 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | TFA- 0.4% |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD50C |