HPLC Method for Separation of Sodium, Potassium, Lithium, and Ammonium on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
Inorganic cations and ammonia are usually analyzed by ion-exchange chromatography with a conductivity detector. Mixed-mode chromatography achieves the same goal with an ELSD detector. In addition to retaining ions, mixed-mode column can retain hydrophobic molecules also, making it possible to analyze a wide variety of ionic and non-ionic compounds in one run. In this method sodium, potassium, and lithium ions were separated along with ammonium ions on a Primesep 100 column. Detection technique is ELSD.
Methodenparameter
| Column | Primesep 100, 4.6 x 250 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | TFA – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |