Separation of Benzoic and Acetylbenzoic acid in Hydrogen-Bonding Mode
A general approach for analysis of various organic acids with MS detection in negative mode was developed using hydrogen-bonding stationary phase – SHARC1. A highly sensitive method allows to analyze traces of organic acids in various matrices using ACN/MeOH/ammonia mobile phase. In hydrogen-bonding chromatography acetonitrile is a weaker solvent and alcohol is a stronger solvent. Various gradient and isocratic conditions can be used
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column . Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Methodenparameter
| Column | Sharc 1, 3.2×100 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/MeOH – 95/5% |
| Buffer | Ammonia 10mM |
| Flow Rate | 005 mL/min |
| Detection | UV, 250 nm |