HPLC Separation of Caffeine, Benzoic acid and Acetaminophen

HPLC-Applikation • Datum: 2015/08/06
HPLC Separation of Caffeine, Benzoic acid and Acetaminophen Chromatogramm

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Methodenparameter

ColumnLegacy L1, 4.6×250 mm, 5 µm, 100 Å
Mobile PhaseMeOH – 28%
BufferAcetic Acid – 3%
Flow Rate1.5 mL/min
DetectionUV, 275 nm