HPLC Separation of Homovanillic Acid, Dopamine, and DOPAC using Hydrogen Bonding
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column . Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Methodenparameter
| Column | Sharc 1, 3.2×50 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | Gradient MeCN/MeOH – 100/0 – 30/70% |
| Buffer | Formic Acid – 0.5% AmFm 0.05% 7 min |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 270 nm |