HPLC-MS Method for Analysis of Lipid Standards: Triglyceride Mixtures on Lipak Column
Series of triglycerides, which are esters derived from glycerol and three fatty acids.
Tricaprylin , Tricaprin , Trimyristin , Trilaurin , Tripalmitin can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of methanol (MeOH), ethanol (EtOH), ammonium formate and formic acid as a buffer. Detection is achieved using LC MS.
Methodenparameter
| Column | Lipak, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeOH/EtOH – 60/40-10/90% in 10 min |
| Buffer | AmFm– 10 mM, FA – 0.05% |
| Flow Rate | 0.5 mL/min |
| Detection | ESI-SIM: [M+NH4]+ – (488, 530, 656, 741, 825); [M + HCOOH + H]+ – (685, 853) |