HPLC MS Method for Analysis of Sodium dodecyl sulfate on Lipak Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium dodecyl sulfate .
Sodium dodecyl sulfate (SDS) , or sodium lauryl sulfate (SLS), is a surfactant used in cleaning, hygienic, and food products. It has a chemical formula C12H25NaSO4 , meaning it has twelve carbon hydrophobic tail attached to a polar sulfate. At low concentrations, it is used as a whipping aid and emulsifier in foods containing egg whites. It has a similar use in pharmaceutics as an ionic solubilizer and, also, an emulsifier. In laboratory uses, it is used as a component for lysing cells during RNA extraction or DNA extraction.
Sodium dodecyl sulfate can be retained and analyzed using the Lipak stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) buffer. Detection is performed using LC MS.
Methodenparameter
| Column | Lipak, 3.2 x 150 mm, 3 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 60/40% – 90/10% |
| Buffer | Ammonium Formate 10 mM, Formic Acid – 0.05% |
| Flow Rate | 0.5 mL/min |
| Detection | LCMSESISIM265 [M-H] – |