ELSD-HPLC Method for Analysis of Phospholipids on Lipak Colulm
Phospholipids are vital components of cell membranes , playing key roles in cell signaling , energy storage , and protective functions . They are essential for maintaining cellular structure and function, making them crucial in biological processes and industrial applications such as drug delivery and cosmetic formulations .
Over time, phospholipids can degrade into various biologically active products , including lysophospholipids and fatty acids , which are involved in cellular signaling and inflammation . Monitoring both phospholipids and their degradation products provides valuable insights into metabolic health , lipid metabolism , and the stability of lipid-based formulations .
Our high-resolution chromatograms ensure accurate and reliable analysis of both phospholipids and their degradation products , supporting applications in clinical diagnostics , pharmaceuticals , and food quality control .
Phospholipids can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of water, methanol (MeOH), ammonium formate and formic acid as a buffer. Detection is achieved using ELSD
Methodenparameter
| Column | Lipak, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeOH –90-100% 20 min, 10 min hold |
| Buffer | AmFm– 10 mM, FA – 0.05% |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 40°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |