HPLC-ELSD Method for Analysis of Lipid Standards: Triglyceride Mixtures on Lipak Column
Series of triglycerides, which are esters derived from glycerol and three fatty acids.
Tricaprylin , Tricaprin , Trilaurin , Trimyristin , Tripalmitin can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of methanol (MeOH), ethanol (EtOH), ammonium formate and formic acid as a buffer. Detection is achieved using ELSD.
Methodenparameter
| Column | Lipak, 3.2 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeOH/EtOH – 50/50-0/100% in 10 min |
| Buffer | AmFm– 10 mM, FA – 0.05% |
| Flow Rate | 0.5 mL/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 40°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |