HPLC Method for Determination of Histamine on BIST A+ Column by SIELC Technologies
Histamine dihydrochloride , also known as Ceplene , is a medication with the chemical formula C5H11Cl2N3 . It is used primarily to prevent a relapse of acute myeloid leukemia. Other pharmaceutical applications include as a topical analgesic to treat arthritis, aches, bruises, sprains, and strains. Brand names it is sold under include Australian Dream and Alo.
Histamine dihydrochloride can be detected in the low UV regime. Using a BIST A+ column and a mobile phase consisting of water and Acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, Histamine chloride can be retained, separated, and analyzed. This analysis method can be detected and is compatible with Mass Spectrometry (MS), ELSD, and CAD.
Methodenparameter
| Column | BIST A+, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 70% |
| Buffer | Ammonium Formate pH 3.0 – 20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD, 50C |