HPLC Method for Analysis of 10A Oligonucleotides on OligoMg Column by SIELC Technologies

Oligo 10-mer oligonucleotides are short sequences of nucleotides consisting of ten nucleotide units. These oligonucleotides can be composed of any combination of the four standard nucleotides: adenine (A), thymine (T), cytosine (C), and guanine (G).

Research and Studies:

• Molecular Biology: In molecular biology, such oligos can be used as primers for PCR, sequencing, or as probes in hybridization experiments.
• Binding Studies: The poly(A) sequence may be used to study binding interactions with proteins, such as DNA-binding proteins or enzymes.

Medical and Diagnostic Applications:

• As part of diagnostic kits, especially in assays that require hybridization to a complementary sequence.

Educational Purposes:

Demonstrating basic principles of nucleic acid chemistry and genetics.

Nanotechnology:

In DNA nanotechnology, specific sequences of DNA are used to form structures and shapes at the nanoscale. A poly(A) sequence might be part of a larger structure.

Using SIELC’s newly introduced BIST™ method, this oligonucleotide can be retained on a OligoMg column. Using this new and unique analysis method, oligonucleotide can be separated, retained, and detected at 260 nm.