HPLC Method For Analysis Of Dopamine and Serotonin on Primesep 200 Column
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Dopamine and Serotonin
Dopamine is a key neurotransmitter and medical stimulant used to treat low blood pressure, low heart rate, and heart attacks. Serotonin is another key neurotransmitter that has pharmaceutical applications – namely as the key ingredient in antidepressant and anti-anxiety medications.These two neurotransmitters can be retained, separated, and analyzed on a Primesep 200 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be UV detected at 280 nm with high resolution and peak symmetry.
Methodenparameter
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 280 nm, |