HPLC Separation of Dopamine and Epinephrine in HILIC and Cation-Exchange Modes
Dopamine and epinephrine were separated on a Primesep S2HILICmixed-mode column. Dopamine and epinephrine are retained by HILIC and cation-exchange mechanisms. Retention time is controlled by the amount of ACN, buffer and buffer pH. Method is compatible with LC/MS and can be used for analysis of polar metabolites in biofluids.
Methodenparameter
| Column | Primesep S2, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 250 nm |