HPLC Separation of Amino Acids in HILIC and Cation-Exchange Modes
Amino acids can be retained and separated on a Primesep S2 column in HILIC/cation-exchange mode.
Methodenparameter
| Column | Primesep S2, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 270 nm |