Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)
Adding on to the previous HPLC separation of amino acids using Bufferless Ion Separation (BLIS) Chromatography; here we have additional amino acids separated on Primesep 200 column using only water and acetonitrile (MeCN, ACN) in the mobile phase. Primesep 200 is a reverse-phase (RP) column with weak acidic ion-pairing groups embedded. With no buffer present in the mobile phase, detection can be achieved with UV, mass spectrometry (MS), evaporative light scattering detection (ELSD).
Methodenparameter
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN |
| Buffer | No |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 195 nm |