HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on BIST B+ Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

Carnosine is a dipeptide molecule synthesized from beta-alanine and histidine. It is a naturally occurring pH buffer with antioxidant properties found in muscles. Using SIELC’s newly introduced BIST™ method, Carnosine can be retained on a positively charged, antion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged peptide to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Carnosine can be separated, retained, and UV detected at 205 nm.