HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
Carnosine is a dipeptide molecule synthesized from beta-alanine and histidine. It is a naturally occurring pH buffer with antioxidant properties found in muscles. This dipeptide can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Trifluoroacetic acid (TFA). This analytical method can be UV detected at 215 nm with high resolution and peak symmetry.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | TFA – 0.6% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 215 nm |