HPLC Method For Analysis Of 2,3-Dichlorobenzoic Anhydride on Primesep S2 Column
Separation type: Liquid Chromatography HILIC
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2,3-Dichlorobenzoic anhydride
2,3-Dichlorobenzoic Anhydride is an impurity formed during the production of Lamotrigine, anticonvulsant used to treat epileptic seizures and Bipolar Disorder. Removing 2,3-Dichlorobenzoic Anhydride is paramount for maximizing Lamotrigine purity. 2,3-Dichlorobenzoic Anhydride can be retained and analyzed on a mixed-mode Primesep S2 column with a mobile phase consisting of just Acetonitrile (MeCN) without water or a buffer. This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
Methodenparameter
| Column | Primesep S2, 4.6×250 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN- 100% |
| Buffer | No |
| Flow Rate | 1.0 mL/min |
| Detection | UV 210 nm |