HPLC ELSD Method for Analysis of Choline on Obelisc R Column
Choline is an essential nutrient that plays a crucial role in various physiological processes in the body. It is often grouped with B-vitamins, but it is not officially recognized as a vitamin. Choline serves several important functions, including:
Food Sources: Choline is found in a variety of foods. Some good dietary sources of choline include:
Choline can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate as a buffer. Detection is achieved using ELSD
Methodenparameter
| Column | Obelisc R, 2.1 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Ammonium acetate pH 5.0 – 10 mM |
| Flow Rate | 0.2 mL/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 70°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Injection Volume | 1 µl |