HPLC ELSD Method for Analysis Spermidine on Obelisc R Column
Spermidine is a polyamine compound that is found in all living cells. It is a biogenic amine and is involved in various cellular processes.
Research and Health Implications:
Spermidine can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD
Methodenparameter
| Column | Obelisc R, 2.1 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Ammonium formate pH 3.0 – 30 mM |
| Flow Rate | 0.2 mL/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Injection Volume | 5 µl |