HPLC ELSD Method for Analysis Butyrylcholine on Obelisc R Column
Butyrylcholine can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD
Methodenparameter
| Column | Obelisc R, 2.1 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | Ammonium formate pH 3.0 – 2 mM |
| Flow Rate | 0.2 mL/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Injection Volume | 1 µl |