ELSD HPLC Method for Analysis of Choline on Primesep 100 Column
Choline is an essential nutrient that plays a vital role in many physiological processes in the body. While it is not classified as a vitamin or mineral, it is often grouped with the B-complex vitamins due to its similar functions.
Sources of Choline: Choline can be obtained through diet and is found in a variety of foods:
Eggs (especially the yolks) Liver (beef and chicken) Fish (salmon and cod) Soybeans and soy products Moderate Sources:
Broccoli Brussels sprouts Peanuts Whole grains Supplements:
Choline supplements, such as choline bitartrate, phosphatidylcholine, or citicoline, are available for those who may have difficulty getting enough through diet alone.
Choline can be retained, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and TFA as a buffer. Detection is carried out using ELSD
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN40% |
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 1 mL/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |