HPLC Determination of Pentadecafluorooctanoic acid (PFOA) on Newcrom R1 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Perfluorooctanoic acid (PFOA) .
Pentadecafluorooctanoic acid , also known as C8 due to it’s chemical being C8HF15O2 , is an industrial surfactant. It is classified by the IARC as a carcinogen to humans. Due to it not breaking down via natural processes, it is also considered a “forever chemical.” It can easily move through soil and contaminate water, leading up to it building up[ in fish and wildlife. Despite that, it has wide use across industries from upholstery, to floor wax, to fire fighting sealants. PFOA was a key point in a renowned legal case of Robert Bilott against DuPont.
Perfluorooctanoic acid (PFOA) can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom R1, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H2SO4 – 0.4% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 220 nm |