USP Methods for the Analysis of Hydrocortisone on a Legacy L1 Column

Application Notes: Hydrocortisone is a naturally occurring hormone released during times of stress. Hydrocortisone has been synthesized and is used to treat diseases such as allergic reactions and skin conditions. According to the USP methods, hydrocortisone contains not less than 97 percent and not more than 102 percent of hydrocortisone calculated on the dried basis. The USP HPLC method for the separation of hydrocortisone was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 µm and pore size of 100-120 Å.

Application Columns: Legacy L1 C18HPLCcolumn

Application compounds: Hydrocortisone

Mobile phase: MeCN/H2O 25:75

Detection technique: UV

Reference: USP30: NF35