Application USP Methods for Nitrofurantoin for the Legacy L1
High Performance Liquid Chromatography (HPLC) Method for Analysis of Nitrofurantoin .
Nitrofurantoin is an oral antibiotic with the chemical formula C8H6N4O5 . It is used to treat urinary tract infections.
Nitrofurantoin can be retained and analyzed using the Legacy L1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Legacy L1, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 43/55% |
| Buffer | NaHPO4pH3.5 – 2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 233 nm |