USP Methods Analysis of Procainamide on Legacy L1 Column

HPLC-Applikation • Datum: 2012/06/21
USP Methods  Analysis of Procainamide on Legacy L1 Column Chromatogramm

Application compounds: Procainamide and 4-aminobenzoic acid

Mobile phase: WAter/MeOH/MeCN (70/70/90) with 7 mM sodium lauryl sulfate and 11mM phosphoric acid

Detection technique: UV

Reference: USP35: NF30

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Methodenparameter

ColumnLegacy L1, 4.6×150 mm, 5 µm, 100 Å
Mobile PhaseWater/MeOH/TEA (140/60/1) adjusted to pH 7.5 with H3PO4
BufferTEAPh
Flow Rate1.5 mL/min
DetectionUV, 230 nm